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Updated: Jul 3, 2026
Purification of a High Molecular Mass Protein in Streptococcus mutans
Published on: September 14, 2019
[Construction of gtfB/CAT eukaryotic expression plasmid of Streptococcus mutans in mammalian cells]
Yun-Peng Qu1, Jian-Guo Liu, De-Qin Yang
1The Affiliated Stomatology Hospital, Wenzhou Medical College. Wenzhou 325027, Zhejiang Province, China. quyunpeng618@163.com
Purpose:
The study aimed to evaluate the expression of recombinant plasmid pVAX1- gtfB/CAT in mammalian COS-7 cells.
Methods:
The eukaryotic plasmid carrying encoding gene of gtfB/CAT of Streptococcus mutans was constructed and introduced into COS-7 cells by lipofectamine reagent. The transient protein expression was detected by immunochemistry technique in COS-7 cells.
Results:
The positive expression of gefB/CAT was detected in plasma of the cells which were transfected with recombinant plasmid pVAX1- gtfB/CAT. The cells which were transfected with pVAX1 were negative for gtfB/CAT expression.
Conclusions:
GtfB/CAT can be translated and expressed in COS-7 cells after transfected with recombinant plasmid pVAX1- gtfB/CAT. The expressed protein is located in the plasma and the protein is able to combine with anti- gtfB/CAT antibody. The expressed protein has the antigenicity and recombinant plasmid pVAX1- gtfB/CAT is a candidate vaccine.Supported by Key Research Project of Bureau of Education of Guizhou Province (Grant No.2004119).
