[HPLC fingerprint of Pinellia ternata]

To establish the HPLC fingerprint of Pinellia ternata (Thunb.) Breit. at Chongqing Municipality for the quality control.

HPLC was used by cosmosil ODS column (4.6 mm x 250 mm, 5 microm) with the gradient elution solvent system composed of MeOH-H2O (0% -5%), the detection wavelength was 254 nm, the sample injection was 20 microl, the flowrate was 1.0 ml/ min, and the temperature of column was 25 degrees C.

HPLC fingerprints of six landing Races of Pinellia ternata (Thunb.) Breit. were established. Eleven mutual peaks were selected as the fingerprint peaks. Among the obtained fingerprints, most of the Detected peaks were separated effectively. The RSDs of the relative retention time and the peak area of mutual peaks, which existed in all samples, less than 0.1% and 1% separately, were in accordance with the request of fingerprint.

The established HPLC fingerprint has desirable accuracy, repeatability and stability, it can be used for one of the quality control of Pinellia ternata (Thunb.). Breit.