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[Study on isolation of Chlorogenic acid from Eucommia ulmoides leaves by ultrafiltration technique]
Zu-Jin Yang1, Yan-Bin Jiang, Fa-Huan Ge
1National Engineering Research Center for Modernization of Extraction and Separation Process of TCM, Guangzhou 510240, China. yangzujin@126.com
Objective:
To isolate and purify Chlorogenic acid from Eucommia ulmoides leaves by ultrafiltration technique.
Methods:
Chlorogenic acid transferring ratio was investigated by temperature, pressure, time, cut-off molecular mass.
Results:
The optimal conditions for the ultrafiltration technique were finally determined. It was found that, for the membrane with a cut-off molecular mass of 20000, when the temperature, pressure, time were respectively 40 degrees C, 0.36 MPa, 20 minutes and adding 24L water, yield of Chlorogenic acid was 99.38%.
Conclusion:
High Chlorogenic acid transfering ratio can be gotten by ultrafiltration technique, which is helpful to the further isolation and purification of Chlorogenic acid in the subsequent process.
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