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Updated: Jul 3, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Exon III splicing of fibroblast growth factor receptor 1 is modulated by growth factors and cyclin D1

Guowei Chen1, Jian Wang, Zhanbing Liu

  • 1Department of General, Visceral, and Transplantation Surgery, University of Ulm, Ulm, Germany.

Pancreas
|July 31, 2008
PubMed
Abstract

Insights

Fibroblast growth factor receptor 1 (FGFR1) isoforms IIIb and IIIc are coexpressed in pancreatic cancer. Growth factors and cyclin D1 differentially regulate these FGFR1 isoforms, impacting cancer cell growth.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Fibroblast growth factor receptor 1 (FGFR1) has distinct isoforms, FGFR1-IIIb and FGFR1-IIIc, with opposing effects on pancreatic cancer cell growth.
  • The expression and regulation of these specific human FGFR1 isoforms remain largely uncharacterized.

Purpose of the Study:

  • To investigate the regulators that modulate the specific expression of human FGFR1-IIIb and FGFR1-IIIc.
  • To understand the differential expression patterns of FGFR1 isoforms in pancreatic cancer.

Main Methods:

  • Utilized parental cells, cells overexpressing FGFR1-III isoforms, and a tetracycline-inducible cyclin D1 antisense system.
  • Analyzed the expression of FGFR1-IIIb and FGFR1-IIIc under varying cell densities and in response to specific growth factors (IGF-I, EGF, FGF1, FGF2, FGF5).

Main Results:

  • FGFR1-IIIb and FGFR1-IIIc were coexpressed in human pancreatic cancer cells, with FGFR1-IIIc being predominant.
  • FGFR1-IIIc expression was induced by IGF-I, EGF, FGF1, FGF2, and FGF5, while FGFR1-IIIb expression was reduced by FGF1, FGF2, and FGF5.
  • Inhibition of cyclin D1 led to decreased FGFR1-IIIc expression and enhanced FGFR1-IIIb expression.

Conclusions:

  • Demonstrated coexpression of FGFR1-IIIb and FGFR1-IIIc in human pancreatic cancer.
  • Established that FGFR1-III isoforms are differentially regulated by specific growth factors and cyclin D1, providing insights into pancreatic cancer progression.

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