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Related Concept Videos

Structure and Function of Platelets01:18

Structure and Function of Platelets

The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...
Formation of the Platelet Plug01:22

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors01:20

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors

Antiplatelet drugs emerge as frontline defenders against the insidious threat of thromboembolic diseases, where abnormal clots obstruct vital blood vessels. These drugs stand as bulwarks, inhibiting platelet aggregation and clot formation, thereby mitigating the risk of life-threatening conditions like myocardial infarction, coronary artery disease, and thrombotic strokes.
Prostaglandin synthesis inhibitors, exemplified by the widely known aspirin, wield their power by irreversibly acetylating...

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Related Experiment Video

Updated: Jul 3, 2026

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry
08:04

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

Effect of dominant negative SNAP-23 expression on platelet function.

A Gillitzer1, M Peluso, A Bültmann

  • 1Trigen AG, Martinsried, Germany.

Journal of Thrombosis and Haemostasis : JTH
|July 31, 2008
PubMed
Summary
This summary is machine-generated.

Platelet protein SNAP-23 is crucial for secretion. Inhibiting SNAP-23 function significantly impaired platelet activation, granule release, and aggregation, confirming its vital role in platelet secretory function.

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Last Updated: Jul 3, 2026

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry
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Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets
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Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation
04:37

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Published on: May 23, 2025

Area of Science:

  • Hematology
  • Cell Biology
  • Molecular Biology

Background:

  • Platelets play a key role in hemostasis and thrombosis.
  • The secretory pathway in platelets involves proteins like SNAP-23.
  • The precise contribution of SNAP-23 to platelet secretion requires further elucidation.

Purpose of the Study:

  • To investigate the role of SNAP-23 in platelet secretory function.
  • To assess the impact of inhibiting SNAP-23 on platelet activation and granule release.

Main Methods:

  • Overexpression of a dominant-negative SNAP-23 mutant in platelets using a novel technology.
  • Analysis of agonist-dependent surface marker expression (P-selectin, CD40L, CD41, CD61).
  • Measurement of dense granule release and platelet aggregation.

Main Results:

  • Dominant-negative SNAP-23 mutant significantly suppressed agonist-dependent surface recruitment of P-selectin and CD40L.
  • Release of dense granule contents was clearly inhibited.
  • Agonist-dependent surface expression of fibrinogen receptor markers (CD41, CD61) was reduced.
  • Platelet aggregation was inhibited.

Conclusions:

  • Inhibition of SNAP-23 function has clear and significant effects on platelet functions.
  • The novel method utilizing recombinant culture-derived platelets enables rapid assessment of protein function in intact platelets.
  • SNAP-23 is functionally important in intact platelets for secretion and activation processes.