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Published on: May 13, 2019
Genome-associated RNA polymerase II includes the dissociable Rpb4/7 subcomplex
Anna J Jasiak1, Holger Hartmann, Elena Karakasili
1Gene Center and Center for Integrated Protein Science Munich CIPSM, Department of Chemistry and Biochemistry, Ludwig-Maximilians-University of Munich, Feodor-Lynen-Strasse 25, D-81377 Munich, Germany.
The Journal of Biological Chemistry
|August 1, 2008
Summary
The Rpb4/7 subcomplex is part of yeast RNA polymerase II in vivo. This complete enzyme associates with DNA, suggesting Rpb4/7 has roles throughout transcription.
Area of Science:
- Molecular Biology
- Yeast Genetics
- Gene Expression
Background:
- Yeast RNA polymerase (Pol) II has a core enzyme and the Rpb4/7 subcomplex.
- The Rpb4/7 subcomplex is dispensable for catalytic activity and dissociates in vitro.
Purpose of the Study:
- To determine if the Rpb4/7 subcomplex is an integral part of DNA-associated Pol II in vivo.
- Investigate the in vivo association of the complete RNA polymerase II with DNA.
Main Methods:
- Chromatin immunoprecipitation (ChIP) was employed.
- High-resolution tiling microarray analysis was used to analyze genome-wide occupancy.
Main Results:
- Genome-wide occupancy profiles for the Rpb7 subunit and the core Rpb3 subunit were identical.
- This indicates that the complete RNA polymerase II, including the Rpb4/7 subcomplex, associates with DNA.
Conclusions:
- The Rpb4/7 subcomplex is an integral part of DNA-associated RNA polymerase II in yeast.
- These findings support functional roles for Rpb4/7 throughout the entire transcription cycle.
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