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Detection of duck plague virus antigen in tissues by immunoperoxidase staining
M R Islam1, J Nessa, K M Halder
1Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Abstract:
An avidin-biotin-peroxidase method of immunoperoxidase staining was successfully adapted for detection of duck plague virus antigen in formalin-fixed, paraffin-embedded tissue sections of the liver and spleen of experimentally infected domestic ducks. Positive staining was localized mostly in the nucleus but was also present in the cytoplasm of a few hepatocytes and von Kupffer cells of the liver, and lymphocytes and reticular cells of the spleen.
