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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

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Related Experiment Video

Updated: Jul 3, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Alternative splicing generates a short BCL6 (BCL6S) isoform encoding a compact repressor.

Yulei Shen1, Baosheng Ge, Himabindu Ramachandrareddy

  • 1Department of Pathology and Microbiology, University of Nebraska Medical Center, 600S. 42th Street, Omaha, NE 68198-7660, USA.

Biochemical and Biophysical Research Communications
|August 5, 2008
PubMed
Summary

Researchers discovered a new BCL6 spliced isoform, BCL6S, which lacks exon 7 but retains key functional domains. This compact repressor may play a role in germinal center B cells.

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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Related Experiment Videos

Last Updated: Jul 3, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Cancer Biology

Background:

  • The BCL6 gene produces multiple mRNA isoforms with distinct transcription start sites.
  • These isoforms typically encode the same BCL6 protein, crucial for gene regulation.
  • Previous studies identified known BCL6 variants, but novel isoforms remained unexplored.

Purpose of the Study:

  • To identify and characterize novel spliced isoforms of the BCL6 gene.
  • To investigate the structure, expression, and function of a newly identified BCL6 isoform, termed BCL6S.
  • To determine the potential role of BCL6S in cellular processes, particularly in germinal center B cells.

Main Methods:

  • Cloning and sequencing of novel BCL6 spliced isoforms.
  • Expression analysis of BCL6S mRNA and protein in human cell lines and tissues.
  • Biochemical assays to assess BCL6S dimerization and DNA-binding capabilities.
  • Luciferase reporter assays to evaluate the transcriptional repressor activity of BCL6S.

Main Results:

  • A novel BCL6 spliced isoform, BCL6S, was identified, lacking exon 7 which encodes the first two zinc fingers.
  • BCL6S mRNA and protein are expressed in human cells and tissues, albeit as a minor component of total BCL6.
  • BCL6S protein can form homodimers and heterodimers with BCL6, bind to BCL6-binding sites, and repress target gene expression.
  • BCL6S functions as a compact transcriptional repressor.

Conclusions:

  • BCL6S represents a novel, functional isoform of the BCL6 gene.
  • This isoform retains DNA-binding and transcriptional repression capabilities, similar to the canonical BCL6 protein.
  • BCL6S may possess a distinct or complementary functional role in normal and neoplastic germinal center B cells.