Related Experiment Video
Updated: Jul 3, 2026

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Published on: December 15, 2017
A statistical approach to optimization of fermentative production of poly(gamma-glutamic acid) from Bacillus
I B Bajaj1, S S Lele, R S Singhal
1Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai, Nathlal Parikh Marg, Matunga, Mumbai 400 019, India. ishbajaj1@yahoo.co.in
Abstract:
This paper reports on the optimization of poly(gamma-glutamic acid) (PGA) production by Bacillus licheniformis NCIM 2324 using a statistical approach. One-factor-at-a-time method was used to investigate the effect of carbon sources, nitrogen sources and pH on PGA production. Plackett-Burman design was adopted to select the most important nutrients influencing the yield of PGA. After identifying effective nutrients, response surface methodology was used to develop a mathematical model to identify the optimum concentrations of the key nutrients for higher PGA production, and confirm its validity experimentally. PGA production increased significantly from 5.27 to 26.12 g/l when the strain was cultivated in the optimal medium developed by using statistical approach, as compared to basal medium.
Related Concept Videos
Bioreactor Controls-III
Production of Organic Acids
Production of Alcohol
Methods of Medium Optimization
Production of Antibiotics
Upstream Processing
