Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distinct dendritic cell subsets are associated with immune dysfunction and clinical severity in sickle cell anemia.

Scientific reports·2026
Same author

The H3K4 methyltransferase KMT2D is an essential cofactor for GATA1 at erythroid gene enhancers.

Nature communications·2026
Same author

Corrigendum to: Laccaic Acid A: A Natural Anthraquinone with Potent Anticancer Activity against MDA-MB-231 Cells.

Current topics in medicinal chemistry·2026
Same author

Cancer During Pregnancy: Navigating Clinical and Research Challenges.

Current oncology (Toronto, Ont.)·2026
Same author

Identification of breakpoint regions and single nucleotide variations of RHD hybrid alleles by long-read sequencing.

Vox sanguinis·2026
Same author

<i>Boesenbergia rotunda</i>-Derived Phytochemicals as Potent Inhibitors of SARS-CoV-2 Papain-Like Protease (PLpro): Insights From Molecular Docking and Dynamic Simulation.

Scientifica·2026

Related Experiment Video

Updated: Jul 3, 2026

Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example
12:44

Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example

Published on: December 3, 2014

An iron responsive element-like stem-loop regulates alpha-hemoglobin-stabilizing protein mRNA.

Camila O dos Santos1, Louis C Dore, Eric Valentine

  • 1Division of Hematology, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA.

The Journal of Biological Chemistry
|August 5, 2008
PubMed
Summary

Iron regulates alpha-hemoglobin-stabilizing protein (AHSP) expression via a novel mechanism involving iron regulatory proteins (IRPs) binding to a non-canonical iron-responsive element (IRE) in AHSP mRNA, impacting hemoglobin synthesis.

More Related Videos

Measurement of Heme Synthesis Levels in Mammalian Cells
09:43

Measurement of Heme Synthesis Levels in Mammalian Cells

Published on: July 9, 2015

Related Experiment Videos

Last Updated: Jul 3, 2026

Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example
12:44

Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example

Published on: December 3, 2014

Measurement of Heme Synthesis Levels in Mammalian Cells
09:43

Measurement of Heme Synthesis Levels in Mammalian Cells

Published on: July 9, 2015

Area of Science:

  • Molecular Biology
  • Hematology
  • Gene Regulation

Background:

  • Hemoglobin synthesis requires precise iron management during erythropoiesis.
  • Alpha-hemoglobin-stabilizing protein (AHSP) is crucial for stabilizing free alpha-globin.
  • Iron homeostasis is critical for red blood cell production.

Purpose of the Study:

  • To investigate the mechanism by which iron regulates AHSP expression.
  • To determine if AHSP mRNA is a target of iron regulatory proteins (IRPs).
  • To elucidate a novel iron-sensing mechanism in hemoglobin production.

Main Methods:

  • Analysis of the 3'-untranslated region (UTR) of primate AHSP mRNA for iron-responsive elements (IREs).
  • Electrophoretic mobility shift assays (EMSAs) to assess IRP binding to the AHSP IRE-like sequence.
  • Co-immunoprecipitation assays to confirm mRNA-protein interactions in cytoplasmic extracts.
  • Assessment of AHSP mRNA stability in erythroid and heterologous cells.

Main Results:

  • AHSP mRNA possesses a unique 3'-UTR stem-loop structure resembling IREs.
  • This IRE-like element binds IRPs, although less efficiently than canonical IREs.
  • IRP binding to AHSP mRNA is iron-dependent and enhances mRNA stability.
  • This interaction occurs in both erythroid and non-erythroid cellular contexts.

Conclusions:

  • IRPs regulate gene expression through non-canonical IREs, expanding known iron-regulated genes.
  • A novel mechanism for modulating hemoglobin production based on iron availability is identified.
  • AHSP represents a new target in understanding iron metabolism during erythropoiesis.