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Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
Published on: November 23, 2016
[Synthesis of diethyl N-[p-(methylamino)benzoyl]-L-glutamate]
Chao Tian1, Shou-xin Zhou, Biao Wang
1Department of Chemical Biology, Peking University School of Pharmaceutical Sciences, Beijing 100083, China.
Objective:
To find a best synthesis method of diethyl N-[p-(methylamino)benzoyl]-L-glutamate(1).
Methods:
Using diethyl N-(p- aminobenzoyl)-L-glutamate (2) as a starting material via a one-pot process of benzylation and methylation, the diethyl N-[p-(methylamino)benzoyl ]-L-glutamate (3) is prepared in high yield. Then compound 1 was conveniently obtained from debenzylation of compound 3 by Pd/C.
Results:
The target compound was obtained through 2-step reaction in 88% overall yield in only 6 h, and its structure was identified by 1HNMR and MS.
Conclusion:
It is a convenient and efficient method of preparation of diethyl N-[p-(methylamino)benzoyl]-L-glutamate.
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