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NiO Nanoflowers for Non-Enzymatic Amperometric Detection of Glucose
Published on: December 30, 2025
Gold nanoparticle-polyaniline composite films for glucose sensing
Pratibha Pandey1, S P Singh, Sunil K Arya
1Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi 110012, India.
Journal of Nanoscience and Nanotechnology
|August 7, 2008
Summary
This study developed a novel glucose biosensor using gold nanoparticles self-assembled on polyaniline films. The resulting biosensor demonstrates rapid detection and linearity for glucose measurement.
Area of Science:
- Electrochemistry
- Nanomaterials Science
- Biotechnology
Background:
- Gold nanoparticles (AuNPs) offer unique electrochemical properties.
- Polyaniline (PANI) is a conductive polymer suitable for biosensor applications.
- Efficient immobilization of enzymes is crucial for biosensor performance.
Purpose of the Study:
- To fabricate a novel glucose biosensor by integrating AuNPs with PANI films.
- To covalently immobilize glucose oxidase (GOx) onto the AuNPs-PANI composite.
- To characterize and evaluate the electrochemical performance of the developed bioelectrode.
Main Methods:
- Self-assembly of AuNPs onto electrochemically deposited PANI films on ITO substrates.
- Covalent immobilization of GOx using EDC/NHS chemistry.
- Characterization via Fourier transform infrared (FTIR) spectroscopy and cyclic voltammetry (CV).
Main Results:
- Successful fabrication of AuNPs-PANI/ITO and GOx/AuNPs-PANI/ITO composite films.
- Enhanced amperometric response current indicating fast electron transfer.
- Achieved a response time of 10 s, linearity from 50 to 300 mg/dl, and Km(app) of 2.2 mM.
Conclusions:
- The developed GOx/AuNPs-PANI/ITO bioelectrode is a promising platform for glucose sensing.
- The integration of AuNPs and PANI enhances electrochemical performance.
- The biosensor exhibits rapid response and good linearity for glucose detection.
