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Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

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Published on: May 18, 2011

Disposable microscope objective lenses for fluorescence correlation spectroscopy using latex microspheres.

Jérôme Wenger¹, Davy Gérard, Heykel Aouani

¹Institut Fresnel, Aix-Marseille Université, CNRS, 13397 Marseille, France.

Analytical Chemistry

|August 7, 2008

Summary

Researchers developed a low-cost optical system using latex microspheres and a low numerical aperture (NA) lens. This system enables sensitive single-molecule detection via fluorescence correlation spectroscopy (FCS) with affordable equipment.

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Conducting Multiple Imaging Modes with One Fluorescence Microscope

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Published on: October 28, 2018

Area of Science:

Optical Physics
Biophysics
Analytical Chemistry

Background:

Fluorescence Correlation Spectroscopy (FCS) is a powerful technique for studying molecular dynamics.
Conventional FCS setups often rely on complex and expensive microscope objectives, limiting accessibility.

Purpose of the Study:

To develop a simple, low-cost optical system for high-performance single-molecule detection.
To demonstrate the feasibility of fluorescence correlation spectroscopy (FCS) using affordable components.

Main Methods:

Combining a latex microsphere with a low numerical aperture (NA) lens to create a novel optical system.
Performing fluorescence correlation spectroscopy (FCS) experiments at various concentrations (1-1000 nM).

Main Results:

Successful detection of single molecules using the developed low-cost optical system.
Demonstrated viability of FCS experiments with objectives costing less than $40.
Achieved high performance comparable to conventional complex objectives.

Conclusions:

The combination of latex microspheres and low NA lenses provides a simple and cost-effective solution for FCS.
This approach significantly lowers the barrier to entry for single-molecule detection experiments.
Offers a viable alternative to expensive, conventional microscopy setups for biophysical studies.