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Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels
Published on: August 7, 2017
Proteomic analysis of undiluted lung epithelial lining fluid
Eric Kipnis1, Kirk Hansen2, Teiji Sawa3
1Infectious Diseases Research Laboratory, Department of Inflammation and Sepsis, Lille University Medical School, Lille, France.
Chest
|August 7, 2008
Summary
Directly recovered undiluted epithelial lining fluid (ELF) using a bronchoscopic microsampling (BMS) probe shows promise for lung disease proteomics. This technique enables biomarker discovery for various lung conditions.
Area of Science:
- Pulmonary Medicine
- Proteomics
- Biomarker Discovery
Background:
- Proteomics is a key tool for discovering biomarkers in human diseases.
- Epithelial lining fluid (ELF) is an attractive but previously indirectly recovered sample for lung disease proteomics.
- Direct recovery of undiluted ELF is now feasible using a bronchoscopic microsampling (BMS) probe.
Purpose of the Study:
- To evaluate the direct recovery of undiluted ELF using a BMS probe in rabbits.
- To assess the proteomic potential of directly recovered ELF for lung disease biomarker discovery.
Main Methods:
- A novel method was developed for non-bronchoscopic probe use and undiluted ELF recovery.
- Recovered ELF underwent 1D-PAGE, fraction digestion, and LC-MS/MS analysis.
- Identified proteins were cross-referenced with Medline for lung disease associations.
Main Results:
- The BMS probe successfully enabled non-bronchoscopic sampling and recovery of undiluted ELF.
- Proteomic analysis revealed numerous proteins in ELF previously linked to lung disease.
- Additional proteins potentially correlated with lung disease were identified.
Conclusions:
- Directly recovered undiluted ELF via BMS probe is a promising sample for lung proteomics.
- This technique holds potential for biomarker discovery in various lung diseases.
- Further studies are required to confirm the application of this sampling technique.