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Updated: Jul 3, 2026

Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays
Published on: December 4, 2016
Sources of endocrine-disrupting chemicals in urban wastewater, Oakland, CA
Jennifer Jackson1, Rebecca Sutton
1East Bay Municipal Utility District, Oakland, CA 94607, USA.
Abstract:
Synthetic endocrine-disrupting chemicals (EDCs) have been found in surface waters throughout the United States, and are known to enter waterways via discharge from wastewater treatment plants (WWTPs). Studies addressing EDCs in wastewater do not examine their specific sources upstream of WWTPs. Presented here are results of a pilot study of potential sources of selected EDCs within an urban wastewater service area. Twenty-one wastewater samples were collected from a range of sites, including 16 residential, commercial, or industrial samples, and five samples from influent and effluent streams at the WWTP. Samples were analyzed for the following known and suspected EDCs: five phthalates, bisphenol A (BPA), triclosan, 4-nonylphenol (NP), and tris(2-chloroethyl) phosphate (TCEP), using well-established methods (EPA 625 and USGS O-1433-01). Twenty of 21 samples contained at least one EDC. Phthalates were widely detected; one or more phthalate compound was identified in 19 of 21 samples. Measurement of two phthalates in a field blank sample suggests that the accuracy of sample detections for these two compounds may be compromised by background contamination. Triclosan was detected in nine samples, BPA in five samples, and TCEP in four samples; NP was not detected. The results of this and future source-specific studies may be used to develop targeted pollution prevention strategies to reduce levels of EDCs in wastewater.
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