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Related Experiment Video

Updated: Jul 3, 2026

Sperm Collection of Differential Quality Using Density Gradient Centrifugation

Sperm Collection of Differential Quality Using Density Gradient Centrifugation

Published on: November 29, 2018

[Sperm morphology does not influence fertilization rate in vitro].

Ying Wu¹, Hui-Ming Xu, Song-Ying Zhang

¹Zhejiang Family Planning Institute, Hangzhou, Zhejiang 310012, China. hz-wuying@163.com

Zhonghua Nan Ke Xue = National Journal of Andrology

|August 9, 2008

Summary

Sperm morphology, including normal forms and defects, does not predict fertilization success in in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). These sperm quality metrics showed no correlation with fertilization or pregnancy rates in assisted reproductive technologies.

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Last Updated: Jul 3, 2026

Sperm Collection of Differential Quality Using Density Gradient Centrifugation

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Published on: November 29, 2018

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

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Fluorimetric Techniques for the Assessment of Sperm Membranes

Fluorimetric Techniques for the Assessment of Sperm Membranes

Published on: November 28, 2018

Area of Science:

Reproductive Biology
Andrology
Infertility Research

Context:

Assisted reproductive technologies (ART) like IVF and ICSI are crucial for treating infertility.
Sperm morphology is traditionally assessed as a factor influencing ART outcomes.
The predictive value of specific sperm morphology parameters, such as the teratozoosperm index (TZI) and sperm deformity index (SDI), requires further clarification.

Purpose:

To investigate the correlation between sperm morphology parameters (normal morphology rate, head defect rate, TZI, SDI) and fertilization outcomes in IVF and ICSI.
To assess if sperm morphology metrics can predict the success of fertilization in assisted reproduction.

Summary:

This study evaluated sperm morphology using Krüger's strict criteria, including normal morphology, head defects, TZI, and SDI.
No significant correlation was found between these sperm morphology parameters and fertilization rates in either IVF or ICSI.
Furthermore, no statistical differences in fertilization, clinical pregnancy, or embryo quality rates were observed between groups stratified by SDI (>1.6 vs. <1.6) or normal sperm morphology (>15% vs. <15%).

Impact:

The findings suggest that traditional sperm morphology assessments may have limited predictive value for fertilization success in IVF and ICSI.
This research may influence clinical decision-making regarding the reliance on sperm morphology parameters in ART protocols.
Further studies may be needed to identify more robust biomarkers for predicting ART outcomes.