Use of Standard Reference Material 2242 (Relative Intensity Correction Standard for Raman Spectroscopy) for...

Area of Science:

• Biotechnology
• Analytical Chemistry
• Metrology

Background:

• Microarray scanner performance is crucial for experimental accuracy but often unquantified.
• Variability and bias introduced by scanners can compromise data integrity in high-throughput biological assays.

Purpose of the Study:

• To develop and validate a method for establishing microarray scanner performance using a Standard Reference Material (SRM).
• To qualify signal measurement and monitor scanner stability in microarray experiments.

Main Methods:

• Utilized Standard Reference Material (SRM) 2,242, certified for Raman spectral correction.
• Monitored microarray fluorescence at 635 nm and 532 nm excitation wavelengths.
• Tracked instrument response, signal intensity, and signal-to-noise ratio (S/N) over time on multiple scanners.

Main Results:

• Demonstrated the utility of SRM 2,242 for monitoring scanner signal stability.
• Confirmed that observed changes in experimental arrays were not due to scanner response fluctuations.
• Showcased the SRM's capability for day-to-day instrument response tracking.

Conclusions:

• SRM 2,242 provides a reliable method for qualifying microarray scanner performance.
• The developed method enables quantification of scanner-induced variability and bias.
• This approach enhances the reliability and reproducibility of microarray experimental data.