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Intranuclear Microinjection of DNA into Dissociated Adult Mammalian Neurons
Published on: December 10, 2009
Femtosecond laser-assisted microinjection into living neurons
Ming Lei1, Hanpeng Xu, Hao Yang
1State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.
Journal of Neuroscience Methods
|August 9, 2008
Abstract:
A Ti:sapphire femtosecond laser micro-manipulation system was used to perforate vital cells. Propidium iodide was successfully transferred into both astrocytes and PC12 cells via the channel of a femtosecond laser, which induced transiently recoverable opening of the cell membrane. The transfection efficiency reached almost 100%. This method also had the advantage of contact-free, non-disruptive and stable transfection.
