High-fidelity compressed high-speed imaging for resolving rapid micro-dynamics

Area of Science:

• Optics and Photonics
• Biomedical Imaging
• Microscopy

Background:

• High-speed optical imaging is crucial for observing fast microscopic events.
• Existing systems face limitations in balancing spatiotemporal resolution, data throughput, and image fidelity.
• Capturing non-repetitive and anisotropic micro-dynamics remains a challenge.

Purpose of the Study:

• To introduce a high-fidelity single-shot compressed high-speed imaging system (HF-CHI).
• To overcome the trade-offs in conventional high-speed imaging for micro-dynamics.
• To enable precise quantitative tracking and observation of rapid microscopic phenomena.

Main Methods:

• Developed HF-CHI by enhancing galvanometer scanning with a high-transmission static encoding mask.
• Employed a multi-prior physics-enhanced network (mPEN) for image reconstruction.
• Integrated dual physical constraints (dynamic sheared measurement and unsheared spatial prior) into the mPEN framework.

Main Results:

• Achieved high spatial resolution (181 lp/mm) and equivalent frame rate (50 Kfps) in a single exposure.
• Successfully tracked single and multiple microsphere trajectories in microfluidics.
• Observed and quantified power-dependent photothermal ablation dynamics in onion epidermal cells, revealing microstructure-guided morphology.

Conclusions:

• HF-CHI provides a robust, high-fidelity solution for single-shot, high-speed micro-dynamics imaging.
• The system demonstrates versatility in microfluidics and laser processing applications.
• Enables detailed investigation of anisotropic micro-dynamics and laser-tissue interactions.