Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 2, 2026

Glycopeptide Capture for Cell Surface Proteomics
10:11

Glycopeptide Capture for Cell Surface Proteomics

Published on: May 9, 2014

Sample preparation method for plasma membrane proteome analysis.

Seung-Ah Park1, Mi-Ryung Kim, Pan-Kyeom Kim

  • 1School of Life Sciences and Biotechnology, Korea University, 1, 5-ga Anam-dong, Sungbuk-ku, Seoul 136-701, Republic of Korea.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|August 12, 2008
PubMed
Summary

Preparing plasma membrane (PM) proteome samples is challenging. A new method using Ultracentrifugation with Percoll and aqueous two-phase extraction offers a rapid, high-purity, and high-yield solution for PM proteome studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recent Progress of Dopamine-Modified Tissue Adhesives for Biomedical Applications in Underwater Environments.

Tissue engineering and regenerative medicine·2026
Same author

Inhibition of Inflammation by an Air-Based No-Ozone Cold Plasma in TNF-α-Induced Human Keratinocytes: An In Vitro Study.

Current issues in molecular biology·2026
Same author

Cell-Cultured Influenza Vaccine Enhances IFN-γ+ T Cell and Memory T Cell Responses Following A/Victoria/2570/2019 IVR-215 (A/H1N1) Infection.

Vaccines·2025
Same author

Highly Resilient Noncovalently Associated Hydrogel Adhesives for Wound Sealing Patch.

Advanced healthcare materials·2024
Same author

Progress of polysaccharide-based tissue adhesives.

Carbohydrate polymers·2024
Same author

A Novel Multi-Component Formulation Reduces Inflammation In Vitro and Clinically Lessens the Symptoms of Chronic Eczematous Skin.

International journal of molecular sciences·2023

Area of Science:

  • Proteomics
  • Cell Biology
  • Biochemistry

Background:

  • Plasma membrane (PM) protein isolation is crucial for cell biology research.
  • PM proteins are hydrophobic and low in abundance, making sample preparation difficult and time-consuming.

Purpose of the Study:

  • To develop an efficient, rapid, and high-purity method for plasma membrane proteome sample preparation.
  • To facilitate downstream analyses such as 2D gel electrophoresis.

Main Methods:

  • Ultracentrifugation with Percoll combined with aqueous two-phase extraction.
  • Optimization of sample preparation for plasma membrane proteins.

Main Results:

  • Achieved a rapid sample preparation time of 3 hours.

More Related Videos

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome
06:31

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome

Published on: March 24, 2023

Related Experiment Videos

Last Updated: Jul 2, 2026

Glycopeptide Capture for Cell Surface Proteomics
10:11

Glycopeptide Capture for Cell Surface Proteomics

Published on: May 9, 2014

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome
06:31

"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome

Published on: March 24, 2023

  • Obtained high purity, with a 26-fold enrichment compared to cell lysate.
  • Secured a high yield, representing 2.6% of whole cell lysate proteins.

    • Conclusions:

    • The developed method provides an efficient and effective approach for plasma membrane proteome analysis.
    • This technique is particularly beneficial for studies utilizing 2D gel electrophoresis.
    • The method addresses the challenges of hydrophobicity and low abundance in PM protein isolation.