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Paneth cell degranulation and lysozyme secretion during acute equine alimentary laminitis
1Department of Veterinary Anatomy and Cellular Biology, College of Veterinary Medicine, Ohio State University, Columbus 43210.
Histochemistry
|January 1, 1991
Summary
Equine Paneth cells release lysozyme during laminitis to combat gut bacteria shifts. This suggests Paneth cells are crucial for the equine intestinal mucosal defense system.
Area of Science:
- Veterinary Science
- Gastroenterology
- Immunology
Background:
- Paneth cells are specialized epithelial cells in the small intestine.
- Their role in equine gastrointestinal health, particularly during metabolic disorders like laminitis, is not fully understood.
- Alimentary laminitis involves significant changes in the gut microbiome.
Purpose of the Study:
- To investigate the response of equine Paneth cells to induced alimentary laminitis.
- To identify the components secreted by Paneth cells during this condition.
- To elucidate the role of Paneth cells in the equine intestinal defense system.
Main Methods:
- Acute alimentary laminitis was induced in mature ponies.
- Gut microbial populations were altered using a carbohydrate-rich diet.
- Paneth cell degranulation and lysozyme presence were assessed using immunohistochemistry.
Main Results:
- A dramatic degranulation of Paneth cells was observed throughout the equine small intestine during acute laminitis.
- Bactericidal lysozyme was identified as a component of Paneth cell secretory granules.
- Lysozyme was found in glandular lumina and degranulated Paneth cells, indicating active secretion.
Conclusions:
- Equine Paneth cells secrete lysozyme in response to carbohydrate-induced gut dysbiosis.
- Lysozyme secretion by Paneth cells appears to be an attempt to regulate the altered microbial population.
- Paneth cells play a significant role in the mucosal defense of the equine intestinal tract.