H2O2-induced changes in astrocytic cultures from control and rapidly aging strains of mouse

The study compared the difference between the H2O2 treatment on astrocytic cultures from a rapidly aging strain of mouse (SAMP8) and its sister control (R1). A mild but statistically significant difference was observed in the numbers of dead cell between R1 and SAMP8 after H2O2 treatment. Cellular changes were equivalent in both strains after injury, including loss of cilia and side projections. Low total dose of H2O2 treatment (e.g., 400 microM for only 1 hour) caused increased cellular synthesis,while high total dose of H2O2 treatment (e.g., 200 microM for 4 hours) downregulated in intracellular synthesis and caused coagulation of microtubules.