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Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension

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Published on: September 11, 2020

Internal calibration to absolute values in flowthrough particle size analysis.

W G Eisert¹, M Nezel

¹Institut für Biophysik, Technische Universität Hannover, Gesellschaft für Strahlen und Umweltforschung, Institut fur Strahlenbotanik, Herrenhäuser Strasse 2, Hannover 21, Germany.

The Review of Scientific Instruments

|December 1, 1978

Summary

This summary is machine-generated.

This study introduces a laser flowthrough microphotometer for precise particle and cell size measurements without external calibration. The innovative device rapidly analyzes particles from 2 to 300 micrometers, orienting nonspherical particles for accurate axis determination.

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Production and Measurement of Organic Particulate Matter in a Flow Tube Reactor

Production and Measurement of Organic Particulate Matter in a Flow Tube Reactor

Published on: December 15, 2018

Area of Science:

Analytical Chemistry
Biophysics
Instrumentation Science

Background:

Accurate particle and cell size measurement is crucial for various scientific disciplines.
Existing methods often require external references or are limited in speed and size range.
Developing advanced microphotometry techniques is essential for high-throughput analysis.

Purpose of the Study:

To describe a novel double-beam laser flowthrough microphotometer.
To enable internal calibrated size measurements of particles and suspensions.
To achieve high-speed, wide-range particle and cell analysis.

Main Methods:

Utilized a double-beam laser flowthrough microphotometer.
Employed light absorption analysis for orienting nonspherical particles and measuring longest axes.
Incorporated light scattering for additional data acquisition.
Validated measurements using latex microspheres and microscopic examination.

Main Results:

The microphotometer allows internal calibrated size measurements without reference standards.
Nonspherical particles are oriented for accurate longest axis measurement via light absorption.
The system analyzes particles in the 2 to 300 micrometer size range.
Analysis rates exceed 100,000 particles/second.
Demonstrated cell classification capabilities with stored human blood samples.

Conclusions:

The developed laser flowthrough microphotometer offers a robust solution for particle and cell size analysis.
Internal calibration and high-throughput capabilities significantly advance microphotometric techniques.
The system demonstrates potential for diverse applications, including biological cell classification.