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Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
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Real-time phase microscopy using a phase-lock interferometer.

D C Leiner1, D T Moore

  • 1Galileo Electro-Optics Corporation, Sturbridge, MA 01518, USA.

The Review of Scientific Instruments
|December 1, 1978
PubMed
Summary
This summary is machine-generated.

A novel phase-lock interference microscope (PLIM) offers real-time optical phase measurement. This advanced instrument achieves high precision, enabling diverse applications in materials science and biology.

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Area of Science:

  • Optical Physics
  • Metrology
  • Microscopy

Background:

  • Accurate optical phase measurement is crucial for characterizing materials and biological samples.
  • Existing methods may lack real-time capabilities or sufficient precision.
  • Interference microscopy offers a non-contact method for phase analysis.

Purpose of the Study:

  • To design and construct a phase-lock interference microscope (PLIM).
  • To enable real-time optical phase measurement with analog output.
  • To demonstrate the PLIM's versatility across various scientific applications.

Main Methods:

  • Development of a phase-lock interference microscope (PLIM) system.
  • Real-time data acquisition of optical phase and position.
  • Application of the PLIM to diverse sample types, including optical fibers, microballoons, biological specimens, and optical surfaces.

Main Results:

  • Successful design and construction of the PLIM instrument.
  • Real-time measurement of optical phase with analog voltage output.
  • Demonstrated capability to measure gradient index profiles, microballoon wall thicknesses, biological specimen phase, and optical surface finish.
  • Achieved a peak-to-peak noise level of lambda/50.

Conclusions:

  • The developed PLIM is a versatile and precise instrument for real-time optical phase measurement.
  • The PLIM's high precision and broad applicability make it valuable for scientific research and quality control.
  • The instrument's performance, particularly its low noise level, meets demanding metrological requirements.