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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Updated: Jul 2, 2026

Production and Multi-Parameter Live Cell Fluorescence Lifetime Imaging Microscopy (FLIM) of Multicellular Spheroids
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ir laser induced fluorescence cell for multiple photon processes.

T J Manuccia1, D S Frankel

  • 1Laser Physics Branch, Optical Sciences Division, Naval Research Laboratory, Washington, DC 20375, USA.

The Review of Scientific Instruments
|March 1, 1978
PubMed
Summary
This summary is machine-generated.

Researchers developed an efficient, easy-to-build long-path laser-induced fluorescence cell. This device aids in studying phenomena induced by multiple infrared photon interactions.

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Area of Science:

  • Physical Chemistry
  • Spectroscopy
  • Laser Physics

Background:

  • Laser-induced fluorescence (LIF) is a sensitive spectroscopic technique.
  • Studying multiple infrared (IR) photon interactions requires specialized experimental setups.
  • Existing methods may lack efficiency or ease of construction.

Purpose of the Study:

  • To describe a novel, efficient, and easily constructed long-path laser-induced fluorescence cell.
  • To present a tool suitable for investigating multiple IR photon-induced phenomena.

Main Methods:

  • Construction of a long-path cell designed for LIF measurements.
  • Integration of laser systems for multiple IR photon excitation.
  • Characterization of the cell's performance in spectroscopic studies.

Main Results:

  • Demonstration of an efficient and user-friendly long-path LIF cell.
  • Successful application of the cell in studies involving multiple IR photon absorption.
  • The cell facilitates detailed analysis of IR-induced processes.

Conclusions:

  • The developed long-path LIF cell is a valuable tool for studying complex photophysical processes.
  • Its ease of construction and efficiency make it accessible for various research applications.
  • This innovation advances the study of multiple IR photon-induced phenomena.