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Expression of metalloproteinases MMP-2 and MMP-9 in antrochoanal polyps
Ozgul Topal1, Selim S Erbek, Halil Kiyici
1Department of Otorhinolaryngology, Baskent University Faculty of Medicine, 06640 Bahcelievler, Ankara, Turkey. ozgultopal75@yahoo.com
Background:
Matrix metalloproteinase (MMP) 2 and MMP-9 are known to cleave specifically type 4 collagen, which is a major structural component of basement membrane. This is an early step of inflammation. Because of this property they have been studied in chronic sinonasal disorders. The aim of this study was to determine the MMP-2 and MMP-9 expressions in an antrochoanal polyp (ACP).
Methods:
We examined tissue samples from 10 ACPs, 10 diffuse nasal polyps, and 10 control nasal mucosa (CM) by immunohistochemistry for MMP-2 and MMP-9 expression.
Results:
Most of epithelial and endothelial cells showed positive immunostaining for MMP-2 and MMP-9 in all tissue samples. MMP-2 staining of inflammatory cells showed no difference among the groups (p > 0.05). On the other hand, MMP-9+ inflammatory cells were found to be significantly increased in ACP and diffuse nasal polyps when compared with CM (p < 0.05).
Conclusion:
MMP-9-expressing inflammatory cells could play a role in the pathophysiology of ACP as well as nasal polyps.
Insights
Matrix metalloproteinase-9 (MMP-9) expressing inflammatory cells are significantly increased in antrochoanal polyps (ACP) and diffuse nasal polyps. These findings suggest MMP-9 plays a role in the pathophysiology of nasal polyps.
Area of Science:
- Biochemistry
- Cell Biology
- Immunology
Background:
- Matrix metalloproteinases (MMPs), specifically MMP-2 and MMP-9, degrade type 4 collagen, a key basement membrane component.
- This degradation is an early inflammatory event, prompting investigation in chronic sinonasal disorders.
Purpose of the Study:
- To investigate the expression of MMP-2 and MMP-9 in antrochoanal polyps (ACP).
Main Methods:
- Immunohistochemistry was used to analyze MMP-2 and MMP-9 expression in tissue samples.
- Samples included 10 ACPs, 10 diffuse nasal polyps, and 10 control nasal mucosa (CM).
Main Results:
- MMP-2 and MMP-9 were expressed in epithelial and endothelial cells across all samples.
- While MMP-2+ inflammatory cells showed no significant difference, MMP-9+ inflammatory cells were significantly elevated in ACP and diffuse nasal polyps compared to controls.
Conclusions:
- Increased MMP-9-expressing inflammatory cells are implicated in the pathophysiology of ACP.
- MMP-9 may contribute to the development of both ACP and diffuse nasal polyps.
