Related Experiment Video
Updated: Jul 2, 2026
Elucidating the Metabolism of 2,4-Dibromophenol in Plants
Published on: February 10, 2023
[Biochemical control of callus maturation--an experimental animal study]
J Schmidt1, H Winker, I Schmidt
1Unfallchirurgie, HELIOS Klinikum Berlin-Buch. joerg.schmidt@helios-kliniken.de
Aim Of The Study:
The purpose of this study was to verify the possibility of monitoring the callus maturation biochemically.
Method:
In an experimental study on sheep, animals with bone fractures with a length 5 cm each were divided into group A (with concomitant damage of the soft tissue) and group B (without soft tissue damage). During callus traction and maturation, biochemical parameters (osteocalcin, AlP, NTx, calcium, phosphate) were measured. Animals of each group (A+B) were euthanised at fixed points in time (according to protocol) and the callus was prepared histologically.
Results:
Total AlP and NTx values were initially reduced during the callus traction and maturation, but increased significantly at the time of Docking+1 transportation time. At the same time, the histological evaluation showed a definite increase of the bone stabilisation, which approximately resembles the structure of a healthy bone as reference.
Conclusion:
With the combination of the AlP and NTx values already determined in the daily clinical routine the monitoring of callus maturation in animal studies becomes possible.
