[Effect of boron and fluoride on the expression of enamelin in rat incisor]
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Updated: Jul 2, 2026
Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
Published on: March 29, 2018
Hong-Mei Wu1, Qiang Wang, Chun-Na Gao
1Department of Operative Dentistry and Endodontics, School of Stomatology, Tongji University, Shanghai 200072, China.
Objective:
To investigate the effects of overdose fluoride, boron and two factors on the expression of enamelin in rat incisor.
Methods:
32 Wistar rats were randomly divided into 4 groups. Group I: The distilled water was given. Group II: 220 mg/L NaF were given. Group III: 382 mg/L Na2B4O2.10H2O were given. Group IV: 220 mg/L NaF and 382 mg/L Na2B4O2.10H2O were given. The rats were sacrificed in the eighth week. HE staining was used to observe the morphology of ameloblasts. Immunohistochemical staining was used for study the expression of enamelin in rat incisors.
Results:
The results showed that the expression of enamelin was reduced in the group II (P<0.01). Compared with group I, the expression of enamelin in group IV had no significant difference. The expression of enamelin in group IV and group II had significant difference (P<0.01).
Conclusion:
The overdose fluoride can inhibit the expression of enamelin. The effection was weaken when boron added. Boron reduced the toxicity of fluoride on teeth.
