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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...

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Updated: Jul 2, 2026

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
14:53

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary

Published on: January 16, 2017

Chiral separation by capillary electromigration techniques.

Gerald Gübitz1, Martin G Schmid

  • 1Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, Karl-Franzens-University Graz, Universitätsplatz 1, A-8010 Graz, Austria. gerald.guebitz@uni-graz.at

Journal of Chromatography. A
|August 19, 2008
PubMed
Summary
This summary is machine-generated.

This review covers chiral separation principles and their applications in capillary electromigration techniques, including capillary zone electrophoresis (CZE) and capillary electrochromatography (CEC). It highlights new chiral selectors and recent advances in separation technologies.

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Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
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Last Updated: Jul 2, 2026

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
14:53

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary

Published on: January 16, 2017

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
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Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

Published on: October 26, 2016

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Chiral Chemistry

Background:

  • Chiral separation is crucial in pharmaceuticals and chemical analysis.
  • Capillary electromigration techniques offer efficient methods for chiral separations.
  • Advances in selectors and technology are continuously improving chiral resolution.

Purpose of the Study:

  • To provide a comprehensive overview of chiral separation principles.
  • To discuss recent developments in capillary zone electrophoresis (CZE) and capillary electrochromatography (CEC).
  • To highlight novel chiral selectors and advanced separation techniques.

Main Methods:

  • Review of fundamental chiral separation principles and mechanisms.
  • Discussion of new chiral selectors including cyclodextrins, crown ethers, and chiral surfactants.
  • Presentation of recent advances in chip technology and detection sensitivity for chiral separations.

Main Results:

  • Explanation of basic chiral separation principles and their mechanisms.
  • Identification of various classes of novel chiral selectors and their recognition abilities.
  • Summary of recent applications in capillary electromigration techniques.

Conclusions:

  • Capillary electromigration techniques are powerful tools for chiral separation.
  • Continuous development of chiral selectors and technologies enhances separation efficiency and sensitivity.
  • The review provides a valuable resource on recent advancements in the field.