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Published on: April 23, 2019
Screening for two selective androgen receptor modulators using gas chromatography-mass spectrometry in doping control
Mario Thevis1, Maxie Kohler, Nils Schlörer
1Center for Preventive Doping Research-Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany. thevis@dshs-koeln.de
European Journal of Mass Spectrometry (Chichester, England)
|August 19, 2008
Summary
Selective androgen receptor modulators (SARMs) show promise for treating diseases. A new gas chromatography-mass spectrometry method effectively detects these compounds in urine for sports drug testing.
Area of Science:
- Pharmacology
- Analytical Chemistry
- Sports Science
Background:
- Selective androgen receptor modulators (SARMs) offer tissue-specific androgen receptor stimulation.
- Clinical research targets SARMs for osteoporosis and frailty, with promising trial results.
- Potential misuse of SARMs in sports necessitates robust drug testing methods.
Purpose of the Study:
- To establish a gas chromatography-mass spectrometry (GC-MS) method for detecting 2-quinolinone- and bicyclic hydantoin-derived SARMs.
- To validate the GC-MS method for use in routine doping control specimens.
Main Methods:
- Urine samples were spiked with SARMs.
- Standard doping control protocols were applied: enzymatic hydrolysis, liquid-liquid extraction, concentration, and derivatization to trimethylsilylated analogues.
- Analysis was performed using gas chromatography and mass spectrometry (GC-MS).
Main Results:
- The GC-MS method demonstrated specificity.
- The lower limit of detection ranged from 0.2 to 10 ng mL(-1).
- Recovery rates were 83-85%, with intraday precision of 9-15% and interday precision of 13-18%.
Conclusions:
- The developed GC-MS method is suitable for detecting 2-quinolinone- and bicyclic hydantoin-derived SARMs in doping control.
- Conventional GC-MS systems can effectively analyze this emerging class of compounds.
- The method supports anti-doping efforts by enabling the identification of SARM misuse in sports.
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