Related Experiment Video
Updated: Jul 2, 2026
Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
Published on: May 27, 2022
Screening of Taxol-producing fungi based on PCR amplification from Taxus
Peng Zhang1, Peng-Peng Zhou, Chen Jiang
1Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, People's Republic of China.
Abstract:
Genes coding for 10-deacetylbaccatin III-10-O-acetyl transferase and C-13 phenylpropanoid side chain-CoA acyltransferase were used as molecular markers for screening of Taxol-producing endophytic fungi. Using PCR, three out of 90 endophytic fungi, isolated from Taxus x media and Taxus yunnanensis, gave positive results. These 3 strains, when grown in 300 ml potato/dextrose liquid medium at 25 degrees C for 10 days, contained 100-160 microg Taxol/g dry wt of mycelium.
