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Characters of DNA constitution in the rye B chromosome
Hong Long1, Zhong-Xia Qi, Xiao-Ming Sun
1College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Abstract:
We have used chromosome microdissection and microcloning to construct a DNA library of the entire B chromosome (B) of rye. New rye B-specific sequences have been screened from this pool, blasted with other sequences and analyzed to elucidate the characters of DNA constitution and the possible pathway of the origin of the rye B chromosome. We report the discovery of a new sequence that is specific to the rye B centromere.
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