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Updated: Sep 2, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
Published on: April 22, 2021
PTI tunes NLR activation via alternative splicing
Ruihua Zhao1,2, Xishi Zhou1, Cui-Jun Zhang1
1State Key Laboratory of Tropical Crop Breeding, Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Abstract:
This Commentary highlights a newly uncovered layer of plant immune regulation, summarizing how surface immune signals license intracellular NLR receptors through alternative RNA splicing. This mechanism enables rapid activation of NLRs upon effector recognition, revealing that surface immunity not only amplifies defense and actively sets the threshold for intracellular immune triggering.
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