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Related Experiment Video

Updated: Jul 2, 2026

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions
08:06

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions

Published on: February 15, 2021

A resource for transcriptomic analysis in the mouse brain.

Charles Plessy1, Michela Fagiolini, Akiko Wagatsuma

  • 1Functional Genomics Technology Team, Omics Science Center, RIKEN Yokohama Institute, Yokohama, Kanagawa, Japan.

Plos One
|August 21, 2008
PubMed
Summary
This summary is machine-generated.

Researchers created a mouse brain cDNA clone collection for transcriptomic analysis. This resource helps study single cell types in the cerebral cortex, revealing over 30% differential gene expression between cell populations.

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Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling

Published on: November 13, 2011

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Last Updated: Jul 2, 2026

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions
08:06

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Published on: February 15, 2021

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Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling
10:06

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling

Published on: November 13, 2011

Area of Science:

  • Neuroscience
  • Genomics
  • Molecular Biology

Background:

  • Cerebral cortex transcriptome is generally homogeneous, masking cell-specific variations.
  • Distinct cell types average out individual population differences, complicating analysis.
  • Transcriptional profiles of sorted cells reveal population-specific differences.

Purpose of the Study:

  • To develop a comprehensive cDNA clone collection for mouse brain transcriptomic studies.
  • To enable the analysis of single cell type transcriptomes in the cerebral cortex.
  • To identify differentially expressed genes between specific neuronal populations.

Main Methods:

  • Generated a low-redundancy set of 16,209 full-length cDNA clones from the mouse visual cortex.
  • Utilized CAGE (CAGE) to confirm cortical expression of 72% of clones.
  • Created microarrays from amplified clones and hybridized with RNA from flow-sorted parvalbumin-EGFP transgenic mouse cortical cells.

Main Results:

  • Developed an annotated cDNA clone collection for mouse brain transcriptomics.
  • Demonstrated differential expression of over 30% of clones between EGFP-positive and EGFP-negative cells.
  • Validated the utility of the clone collection for cell-type specific transcriptomic analysis.

Conclusions:

  • The annotated cDNA clone collection is a valuable resource for mouse brain transcriptomic research.
  • This resource facilitates the study of single cell type transcriptomes in the cerebral cortex.
  • The findings highlight significant transcriptional heterogeneity among cortical cell types.