Related Experiment Video
Updated: Jul 2, 2026

Isolation of Primary Mouse Retinal Glial Müller Cells
Published on: August 30, 2024
Müller cells do not influence leukocyte adhesion to retinal endothelial cells
Ermelindo C Leal1, Célia A Aveleira, Aurea F Castilho
1Center of Ophthalmology, IBILI, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.
Purpose:
Diabetic retinopathy is associated with inflammation. The authors investigated the influence of Müller cells on leukocyte adhesion to retinal endothelial cells.
Methods:
ICAM-1 levels were assessed by Western blotting and immunocytochemistry. Leukocyte adhesion was quantified using a fluorescence assay.
Results:
High glucose and oxidative/nitrosative stress conditions increased ICAM-1 levels in endothelial cells and leukocyte adhesion. Under the influence of Müller cells (co-cultures/conditioned medium), the effects were comparable to those found when endothelial cells were exposed, alone, to similar conditions.
Conclusions:
These results show that Müller cells do not influence leukocyte adhesion under the in vitro conditions used in this study.
Related Concept Videos
Immunoglobulin-like Cell Adhesion Molecules
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Selectins

