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Updated: Jul 2, 2026
14:23
A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
Published on: June 6, 2018
High-quality binary protein interaction map of the yeast interactome network
Haiyuan Yu1, Pascal Braun, Muhammed A Yildirim
1Center for Cancer Systems Biology (CCSB), Dana-Farber Cancer Institute, Boston, MA 02115, USA.
Science (New York, N.Y.)
|August 23, 2008
Summary
This study reveals high-quality binary protein interactions in yeast using yeast two-hybrid (Y2H) screening. The new interactome map highlights transient signaling and connections between essential proteins, correlating connectivity with genetic pleiotropy.
Area of Science:
- Molecular Biology
- Systems Biology
- Bioinformatics
Background:
- Current yeast interactome maps have limited and debated direct binary interactions.
- High-throughput yeast two-hybrid (Y2H) screening offers high-quality binary interaction data.
- A significant portion of the yeast binary interactome remains unmapped.
Purpose of the Study:
- To assess the quality of existing yeast interactome datasets.
- To develop a framework for a high-quality, second-generation yeast binary interactome map.
- To compare the properties of binary interaction networks with co-complex network models.
Main Methods:
- Comparative quality assessment of yeast interactome datasets.
- Development of an empirically controlled mapping framework for Y2H screening.
- Generation of a large-scale Y2H dataset covering ~20% of yeast binary interactions.
- Analysis of topological and biological properties of Y2H and affinity purification followed by mass spectrometry (AP/MS) networks.
Main Results:
- Yeast two-hybrid (Y2H) screening provides high-quality binary interaction information.
- Y2H and AP/MS data are of comparable quality but fundamentally different and complementary.
- The new binary map is enriched for transient signaling interactions and intercomplex connections.
- Protein connectivity in the binary map significantly clusters essential proteins and correlates with genetic pleiotropy, not essentiality.
Conclusions:
- High-throughput Y2H is a valuable method for mapping binary protein interactions.
- Binary interaction maps offer complementary insights to co-complex networks, particularly for signaling pathways.
- Protein connectivity patterns reveal fundamental aspects of cellular organization and function, such as genetic pleiotropy.
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