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Updated: Jul 2, 2026

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
Published on: May 26, 2013
[Isolation and physiological characteristics of lytic bacteriophages of Vibrio]
Jia Sun1, Dezan Ye, Agnes Kochel
1Third Institute of Oceanography, Xiamen 361005, China. oscar_0406@163.com
Objective:
Vibrio is a widely distributed pathogen in aquatic environment. Our study aimed at searching for possible biological control of pathogenic vibrio.
Methods:
We collected natural samples from coast and lakes in spring of 2006 and autumn of 2005; and isolated lytic phages by double-layer plate method. We identified the hosts with 16S rDNA sequencing and observed their morphology with phages under electron microscopy. We also tested the physiological characteristics of phages.
Results:
We isolated 96 bacteria and 2 phages (Vibio/XM/P1, Vibio/XM/P2). Their hosts belonged to Vibrio alginolyticus and Vibrio anguillarum. Both phages were hexagonal-headed and one with a tail. Physiological tests show that their optimum grow condition were pH7, 25 degrees C and pH8, 30 degrees C. Both phages were sensitive to high temperature and UV light. Vibrio/XM/P2 was sensitive to aether and chloroform whereas Vibrio/XM/P1 not.
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