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Novel Probiotic Lactic Acid Bacteria Were Identified from Healthy Infant Feces and Exhibited Anti-Inflammatory

Binbin Li1,2, Li-Long Pan3, Jia Sun1,2

  • 1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Antioxidants (Basel, Switzerland)
|July 27, 2022
PubMed

Insights

Lactic acid bacteria from infant feces show probiotic potential. Certain strains, especially Lactobacillus gasseri FWJL-4, exhibit antioxidant and anti-inflammatory properties, suggesting therapeutic applications for oxidative stress-related diseases.

Area of Science:

  • Microbiology
  • Immunology
  • Biochemistry

Background:

  • Oxidative stress contributes to various diseases.
  • Lactic acid bacteria (LAB) are known for their health benefits.
  • Probiotics offer a potential therapeutic avenue for managing oxidative stress.

Purpose of the Study:

  • To evaluate the probiotic potential of LAB isolated from infant feces.
  • To identify LAB strains with antioxidant and anti-inflammatory properties.
  • To explore the therapeutic applications of these LAB in oxidative stress-related diseases.

Main Methods:

  • In vitro probiotic attribute testing and 16S rRNA genotypic characterization.
  • Assessment of anti-inflammatory effects using real-time quantitative PCR (RT-qPCR).
  • Evaluation of anti-oxidative effects via Western blot analysis in RAW 264.7 macrophages.

Main Results:

  • Selected LAB strains identified as Lactobacillus gasseri, Lactiplantibacillus plantarum, and Lacticaseibacillus rhamnosus.
  • L. gasseri FWJL-4, L. plantarum Fjias-5, and L. rhamnosus FSJ-13 significantly down-regulated pro-inflammatory cytokines (TNFα, IL-6, IL-1β).
  • Treated macrophages showed enhanced antioxidant enzyme activity, reduced hydrogen peroxide (H2O2) production, and increased expression of Nrf2 and HO-1.

Conclusions:

  • Lactobacillus gasseri FWJL-4, L. plantarum Fjias-5, and L. rhamnosus FSJ-13 demonstrate significant antioxidant and anti-inflammatory effects.
  • L. gasseri FWJL-4 is a promising candidate for further in vivo studies.
  • These LAB strains hold potential for developing functional foods or therapeutics for oxidative stress-related conditions.

Keywords:
anti-inflammationantioxidantsinfant fecesisolationlactic acid bacteriaprobiotic

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