Corneal abnormalities in the NC/Nga mouse: an atopic dermatitis model

Area of Science:

• Ophthalmology
• Dermatology
• Immunology

Background:

• Atopic dermatitis (AD) is a chronic inflammatory skin disease.
• NC/Nga mice are a recognized animal model for studying AD.
• Ocular manifestations of AD are common but require further investigation in animal models.

Purpose of the Study:

• To investigate corneal abnormalities in NC/Nga mice.
• To characterize the ocular pathology associated with atopic dermatitis in this model.
• To explore the role of eye-scratching behavior in the development of corneal disorders.

Main Methods:

• Histopathological examination of eyelids, conjunctiva, and corneas using light and electron microscopy.
• Immunohistochemistry and Transferase-mediated dUTP nick-end labeling (TUNL) staining for apoptotic cells.
• Video recording to assess eye-scratching behavior and the efficacy of tacrolimus hydrate ointment.

Main Results:

• NC/Nga mice developed blepharoconjunctivitis characterized by allergic inflammation.
• Severe cases showed corneal epithelial thinning, stromal neovascularization, and cone-shaped corneas.
• Increased apoptotic cells were observed in the corneal epithelium and keratocytes.
• Tacrolimus hydrate ointment effectively reduced eye-scratching behavior.

Conclusions:

• NC/Nga mice serve as a valuable model for atopic blepharoconjunctivitis.
• Corneal abnormalities in this model are likely linked to compulsive eye-scratching behavior.
• Understanding these ocular changes is crucial for managing AD-related eye complications.