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Updated: Jul 2, 2026

The Multifaceted Benefits of Protein Co-expression in Escherichia coli
Published on: February 5, 2015
Expression of human transforming growth factor beta1 in E. coli (I) : Direct expression in cytoplasm
Abstract:
PCR technique is used to amplify the mature peptide gene of human transforming growth factor pl (hTGFbeta1); the gene is verified by full-length sequence analysis. In DHSalpha/pBV220 expression system, hTGFbeta1 attains expression in the cytoplasm of E. coli up to 16%. The recombinant protein is proved to be the monomer of hTGFbetal by N-terminal amino acids analysis and immunoblotting. After refolding of the monomer protein in vitro in glutathione system or CHPAS/DMSO system, the dimeric protein accumulates to 30% in the refolding mixture. The recombinant protein is purified to homogeneity on silver staining, and is shown to have strong biological activity from MTT bioassay on MvlLu cells.
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