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Updated: Jul 2, 2026

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
Published on: January 13, 2017
Diverse bacterial PKS sequences derived from okadaic acid-producing dinoflagellates
Roberto Perez1, Li Liu, Jose Lopez
1Department of Chemistry and Biochemistry, Florida International University, Miami, FL 33199, USA. rperez@med.miami.edu
Roseobacter bacteria are the most abundant microbes found with okadaic acid (OA)-producing Prorocentrum dinoflagellates, suggesting a potential role in toxin synthesis. Their polyketide synthase genes were not directly linked to OA production in these dinoflagellates.
Area of Science:
- Marine microbiology
- Phycology
- Biochemistry
Background:
- Okadaic acid (OA) and related dinophysistoxins are marine algal toxins.
- Bacteria from the Roseobacter group have been previously linked to OA production.
Purpose of the Study:
- To investigate the bacterial community associated with OA-producing dinoflagellates.
- To explore the potential role of Roseobacter bacteria in OA biosynthesis.
Main Methods:
- Analysis of 16S rRNA gene libraries from dinoflagellate samples.
- Investigated polyketide synthase (PKS) gene presence and relationships.
Main Results:
- Roseobacter bacteria were the most abundant in OA-producing Prorocentrum dinoflagellates.
- These Roseobacter were absent from non-toxic dinoflagellate associations.
- Most bacterial PKS genes identified were unrelated to known OA-related or dinoflagellate PKS genes.
Conclusions:
- Roseobacter group bacteria are strongly associated with OA-producing Prorocentrum dinoflagellates.
- The genetic basis for Roseobacter involvement in OA production remains unclear and requires further investigation.
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