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Related Experiment Videos

Polymorphonuclear leukocyte degranulation with exposure to polymethylmethacrylate nanoparticles.

F J Papatheofanis1, R Barmada

  • 1Department of Orthopaedics, University of Illinois, College of Medicine, Chicago 60680.

Journal of Biomedical Materials Research
|June 1, 1991
PubMed
Summary

Polymethylmethacrylate (PMMA) cement triggers inflammatory responses by causing polymorphonuclear leukocytes (PMNs) to release lysosomal contents. This study shows PMMA particles increase PMN degranulation and decrease their migration, explaining tissue reactions.

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Area of Science:

  • Biomaterials Science
  • Immunology
  • Orthopaedic Surgery

Background:

  • Polymethylmethacrylate (PMMA) is widely used in orthopaedic surgery.
  • The inflammatory reaction to PMMA in tissues is not fully understood.
  • Polymorphonuclear leukocytes (PMNs) are key immune cells involved in inflammatory responses.

Purpose of the Study:

  • To investigate the effect of PMMA particles on human PMNs.
  • To determine if PMMA induces degranulation and affects PMN migration.
  • To elucidate the cellular mechanisms behind PMMA-induced inflammation in orthopaedics.

Main Methods:

  • Human PMNs were incubated with varying concentrations of PMMA particles (50-60 nm).
  • Release of lactate dehydrogenase, lysozyme, and beta-glucuronidase from PMNs was measured.

Related Experiment Videos

  • PMN migration assays were performed following exposure to PMMA.

    • Main Results:

    • PMMA particles caused a dose-dependent increase in the release of lysosomal enzymes from PMNs.
    • Exposure to increasing amounts of PMMA significantly reduced PMN migration.
    • These findings indicate PMMA directly stimulates inflammatory cell responses.

    Conclusions:

    • PMMA particles stimulate the degranulation of human PMNs, releasing inflammatory mediators.
    • PMMA alters PMN migration, potentially contributing to the foreign body response.
    • Understanding these cellular interactions is crucial for managing PMMA-related inflammation in orthopaedic implants.