Adamantane/beta-cyclodextrin affinity biosensors based on single-walled carbon nanotubes

Area of Science:

• Electrochemistry
• Materials Science
• Biosensor Development

Background:

• Developing three-dimensional (3D) biostructures on electrode surfaces is a significant challenge in biosensor technology.
• Single-walled carbon nanotubes (SWCNTs) offer a promising framework for creating advanced biosensor architectures.
• Efficient immobilization of enzymes like glucose oxidase (GOX) is crucial for biosensor performance.

Purpose of the Study:

• To synthesize a novel adamantane-pyrrole monomer for electrochemical polymerization.
• To construct 3D SWCNT-based biosensor architectures for enhanced glucose detection.
• To investigate the performance of a supramolecular affinity system for enzyme immobilization.

Main Methods:

• Electrochemical polymerization of adamantane-pyrrole monomer to form a polypyrrole film.
• Functionalization of SWCNT coatings with poly(adamantane-pyrrole) for enzyme anchoring.
• Utilizing an adamantane-cyclodextrin affinity system with beta-cyclodextrin-modified gold nanoparticles as an intermediate layer for GOX immobilization.
• Characterization of supramolecular assemblies using scanning electron microscopy (SEM).
• Electrochemical measurements to assess biosensor response to glucose.

Main Results:

• The developed 3D SWCNT-based biosensor functionalized with poly(adamantane-pyrrole) and employing a beta-cyclodextrin-modified gold nanoparticle intermediate layer achieved high sensitivity (31.02 mAM(-1)cm(-2)) and maximum current density (350 microAcm(-2)).
• The configuration without SWCNTs showed significantly lower performance (0.98 mAM(-1)cm(-2) sensitivity and 75 microAcm(-2) J(max)).
• The adamantane-cyclodextrin supramolecular interaction effectively facilitated the immobilization of adamantane-tagged glucose oxidase.

Conclusions:

• The novel adamantane-pyrrole monomer and SWCNT framework enable the creation of robust 3D biostructures for biosensor applications.
• The beta-cyclodextrin-modified gold nanoparticle intermediate layer significantly enhances biosensor performance for glucose detection.
• This study provides a detailed comparison of different preparation methods and affinity sensor setups, highlighting the advantages of the proposed 3D architecture.