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Updated: Jul 2, 2026
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Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue
Published on: June 14, 2020
Chronic tonsillitis: histological and immunohistochemical aspects.
Carmen Aurelia Mogoantă1, Elena Ioniţă, D Pirici
1ENT Department, University of Medicine and Pharmacy of Craiova, Craiova, Romania. carmen_mogo@yahoo.com
Summary
This study investigated chronic tonsillitis using histology and immunohistochemistry. While B-lymphocytes typically populate germinal centers, T-lymphocytes can predominate in some pathological cases of chronic tonsillitis.
Area of Science:
- Pathology
- Immunohistochemistry
- Otorhinolaryngology
Background:
- Chronic tonsillitis is a common pharyngeal inflammatory condition.
- It can lead to various local and distant complications.
- Histological and immunohistochemical analysis provides insight into its pathogenesis.
Purpose of the Study:
- To investigate the histological and immunohistochemical features of chronic tonsillitis.
- To analyze the cellular composition of tonsillar lymphoid follicles in chronic tonsillitis.
Main Methods:
- 112 tonsil samples from 56 chronic tonsillitis patients were analyzed.
- Histological staining included Hematoxylin-Eosin, trichromic, and argental impregnation.
- Immunohistochemistry used CD20, CD45 RO, and CD68 antibodies to identify T-lymphocytes, B-lymphocytes, and macrophages.
Main Results:
- Hyperplasia and hypertrophy of lymphoid follicles with prominent germinal centers were observed.
- Microhemorrhages and collagen-rich stroma were noted in some samples.
- Immunohistochemistry confirmed B-lymphocytes in germinal centers and T-lymphocytes in cortical regions and epithelium.
Conclusions:
- The study elucidated the cellular dynamics within tonsillar lymphoid follicles in chronic tonsillitis.
- While B-lymphocytes are typical, T-lymphocytes can be the predominant cell type in germinal centers in certain pathological instances.
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