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Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
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Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

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Junction probes - sequence specific detection of nucleic acids via template enhanced hybridization processes

Shizuka Nakayama1, Lei Yan, Herman O Sintim

  • 1Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742, USA.

Journal of the American Chemical Society
|September 2, 2008
PubMed
Summary

Junction probe technology enables amplified nucleic acid detection at constant temperatures. Adding a second dimension to probes allows inexpensive enzymes to detect single nucleotide polymorphisms (SNPs).

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