Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

METTL3 regulates exocytosis independently of m<sup>6</sup>A.

Science advances·2026

Same author

Salmonella lipopolysaccharide-containing supported lipid bilayers as platforms to study bacteriophage interactions.

FEBS letters·2026

Same author

Protocol for total internal reflection fluorescence microscopy image preprocessing and radial analysis of LFA-1 in T cell immune synapses.

STAR protocols·2026

Same author

Advancing physical virology through multiscale approaches-Insights from the 2025 FEBS|EMBO lecture course 'Physical Virology: across length scales'.

FEBS letters·2026

Same author

Multivalency influences virus dynamics at the glycocalyx: A study of glycosaminoglycan binding in HPV16 entry.

Biophysical journal·2026

Same author

An integrated <i>i</i> <i>n vitro</i> platform and biophysical modeling approach for studying synaptic transmission in isolated neuronal pairs.

iScience·2026

Same journal

Four-in-one multifunctional CoCu-NC@AuPt nanozyme integrated M13 phage-displayed nanobody based multimodal lateral flow immunoassay for bovine lactoferrin detection.

Biosensors & bioelectronics·2026

Same journal

A novel capillary-driven dual-mode imaging flow cytometry system for malaria parasite detection and quantification.

Biosensors & bioelectronics·2026

Same journal

Liver-targeted alkaline phosphatase-activatable fluorescent probe for imaging liver fibrosis and screening anti-fibrotic natural products.

Biosensors & bioelectronics·2026

Same journal

GLASS-seq: a gel-anchored, ligation-assisted, scalable biosensing platform for low-cost regional spatial transcriptomics.

Biosensors & bioelectronics·2026

Same journal

CRISPR/Cas12a-based dual-modal signal platform using MIL-101(Fe) for colorimetric and electron spin resonance detection of HPV-16 nucleic acid.

Biosensors & bioelectronics·2026

Same journal

Fully automated centrifugal microfluidic system for self-calibrating isothermal nucleic acid quantification.

Biosensors & bioelectronics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 2, 2026

Fluorescence detection methods for microfluidic droplet platforms

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Particle flow assays for fluorescent protein microarray applications.

Marta Bally¹, Raghuram Dhumpa, Janos Vörös

¹Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH and University of Zurich, Gloriastrasse 35, Zurich, Switzerland.

Biosensors & Bioelectronics

|September 2, 2008

Summary

This summary is machine-generated.

This study introduces a novel fluorescent microparticle assay for enhanced signal amplification in bioanalytical microarrays. This generic approach improves sensitivity and accuracy, enabling visualization of single biomolecular interactions.

More Related Videos

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Published on: August 15, 2013

High-throughput Protein Expression Generator Using a Microfluidic Platform

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

Related Experiment Videos

Last Updated: Jul 2, 2026

Fluorescence detection methods for microfluidic droplet platforms

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Published on: August 15, 2013

High-throughput Protein Expression Generator Using a Microfluidic Platform

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

Area of Science:

Bioanalytical Chemistry
Biotechnology
Microarray Technology

Background:

Microarray technology has revolutionized bioanalysis, but enhanced sensitivity and accuracy are crucial for further breakthroughs.
Current assays often require high-performance equipment and struggle with non-specific binding.

Purpose of the Study:

To develop a simple, generic fluorescent signal amplification method for microarrays.
To demonstrate the feasibility of using fluorescent microparticle labels for enhanced detection.

Main Methods:

A reverse array model was used with biotinylated bovine serum albumin spots.
Streptavidin-coated fluorescent microparticles were applied under controlled flow for specific binding.
Confocal microscopy was employed for visualization and quantification of bound microparticles.

Main Results:

Non-specific particle binding was reduced to less than 1 particle/spot, allowing visualization of single biomolecular bonds.
The amplification scheme is generic and applicable to any fluorescent microarray.
The assay utilizes a biotin-streptavidin linkage, suitable for diverse applications.

Conclusions:

The developed fluorescent microparticle assay offers a simple, generic, and highly sensitive method for microarray analysis.
This approach significantly reduces non-specific binding and enables visualization of single molecular interactions.
The assay's compatibility with standard optical equipment makes it widely accessible.