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A planar interdigitated ring electrode array via dielectrophoresis for uniform patterning of cells
Lo-Chang Hsiung1, Chun-Hui Yang, Chi-Li Chiu
1Institute of Applied Mechanics, National Taiwan University, Taipei 106, Taiwan, ROC.
Biosensors & Bioelectronics
|September 2, 2008
Summary
This study introduces a collagen-coated planar interdigitated ring electrode (PIRE) array for uniform cell patterning using dielectrophoresis. The PIRE array enables rapid, reproducible, and uniform cell distribution for biosensor applications.
Area of Science:
- Biomedical Engineering
- Cell Biology
- Microfluidics
Background:
- Uniform cell patterning is crucial for in vitro studies of cell-cell interactions.
- Achieving consistent cell distribution in vitro presents significant challenges.
Purpose of the Study:
- To develop a novel collagen-coated planar interdigitated ring electrode (PIRE) array.
- To utilize positive dielectrophoresis for uniform cell patterning.
- To enhance cell-based biosensor performance and reproducibility.
Main Methods:
- Designed PIREs with optimized electrode geometry to maximize electric field strength.
- Employed positive dielectrophoresis for cell manipulation.
- Coated PIREs with collagen to promote cell adhesion.
- Tested the system using human hepatocellular carcinoma cells (HepG2).
Main Results:
- Achieved uniform HepG2 cell patterning within minutes on a 6x6 PIRE array (48+/-6 cells per PIRE).
- Demonstrated high cell viability (>24h) and confinement to collagen-coated PIREs.
- Showed acceptable reproducibility in cell viability across PIREs (mean normalized intensity 1+/-0.138).
Conclusions:
- The PIRE array effectively achieves uniform cellular patterning.
- This technology is beneficial for applications requiring consistent cell distribution.
- The PIRE array improves the response and reproducibility of cell-based biosensors.
