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Updated: Jul 2, 2026

Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast

Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast

Published on: June 23, 2018

Protein:protein aggregation induced by protein oxidation.

Hamid Mirzaei¹, Fred Regnier

¹Department of Chemistry, Purdue University, West Lafayette, IN 47907-2084, USA.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

|September 2, 2008

Summary

Oxidative stress damages cells, causing protein aggregation. This study identified 25 proteins forming aggresomes in yeast, offering insights into aging and disease mechanisms.

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Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast

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Area of Science:

Biochemistry
Cell Biology
Proteomics

Background:

Reactive oxygen species (ROS) can overwhelm cellular defenses, leading to damage and protein aggregation.
Protein aggregates are implicated in various diseases and aging processes.

Purpose of the Study:

To investigate the dynamics of oxidative stress-induced protein aggregation.
To identify proteins that form aggregates in vivo under oxidative stress.

Main Methods:

Oxidation of model proteins and analysis via size exclusion chromatography (SEC).
Hydrogen peroxide stress in yeast, followed by LC/MS analysis of high molecular weight aggregates.
Differential labeling of aggregated proteins using isotope-coded N-acetoxysuccinamide.

Main Results:

Oxidation causes protein aggregation in stages, with fragments also forming aggregates.
Twenty-five proteins were identified as part of high molecular weight aggresomes in stressed yeast.
Fifteen of these aggresome proteins showed evidence of carbonylation.

Conclusions:

The study elucidates the multi-stage process of oxidative stress-induced protein aggregation.
Identification of aggresome components provides potential targets for understanding and treating age-related diseases.