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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jul 2, 2026

Lensless Fluorescent Microscopy on a Chip

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

On-chip fluorescence-activated particle counting and sorting system.

Yuejun Kang¹, Xudong Wu, Yao-Nan Wang

¹Department of Mechanical Engineering, Vanderbilt University, Nashville, TN 37235, USA.

Analytica Chimica Acta

|September 2, 2008

Summary

This summary is machine-generated.

A new fluorescence-activated system for lab-on-a-chip applications enables automatic particle counting and sorting. This affordable and portable prototype utilizes electrokinetic flow switching for precise sample manipulation.

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Last Updated: Jul 2, 2026

Lensless Fluorescent Microscopy on a Chip

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Area of Science:

Biomedical Engineering
Analytical Chemistry
Microfluidics

Background:

Lab-on-a-chip (LOC) devices require efficient particle manipulation.
Existing flow cytometry instruments can be expensive and bulky.
Automated particle sorting and counting are crucial for LOC applications.

Purpose of the Study:

To develop a fluorescence-activated particle counting and sorting system for LOC applications.
To create an integrated system combining microfluidics, fluorescence detection, and electronic control.
To design a user-friendly prototype for affordable and portable flow cytometry.

Main Methods:

Integration of a microfluidic chip with fluorescence excitation, detection, and optical visualization.
Utilizing electrokinetic flow switching triggered by fluorescence thresholds for automatic sorting.
Employing direct current electric pulses for dispensing sorted particles.
Development of a user-friendly software interface for real-time control and data display.

Main Results:

Successful development of a fluorescence-activated particle counting and sorting system.
Demonstration of automatic sorting based on pre-set fluorescence levels.
Creation of a simple, disposable microfluidic chip for easy integration.
Development of a software interface for real-time counting, sorting, and visualization.

Conclusions:

The developed system is a promising prototype for affordable and portable flow cytometric instruments.
The integrated approach offers efficient particle counting and sorting capabilities on a chip.
The system's user-friendly design and disposable chip facilitate practical applications.