Related Experiment Video
Updated: Jul 2, 2026

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
Synthesis of DNA-organic molecule-DNA triblock oligomers using the amide coupling reaction and their enzymatic
Jungkyu K Lee1, Young Hwan Jung, Randall M Stoltenberg
1Department of Chemistry, Stanford University, Stanford, California 94305, USA.
Abstract:
Precise electrical contact between single-molecule and electrodes is a first step to study single-molecule electronics and its application such as (bio)sensors and nanodevices. To realize a reliable electrical contact, we can use DNA as a template in the field of nanoelectronics because of its micrometer-scaled length with the thickness of nanometer-scale. In this paper, we studied the reactivity of the amide-coupling reaction to tether oligodeoxynucleotides (ODNs) to organic molecules and the elongation of the ODNs by the polymerase chain reaction (PCR) to synthesize 1.5 kbp dsDNA-organic molecule-1.5 kbp dsDNA (DOD) triblock architecture. The successful amide-coupling reactions were confirmed by electrospray ionization mass spectrometry (ESI-MS), and the triblock architectures were characterized by 1% agarose gel electrophoresis and atomic force microscope (AFM). Our result shows that this strategy is simple and makes it easy to construct DNA-organic molecule-DNA triblock architectures and potentially provides a platform to prepare and investigate single molecule electronics.
Related Concept Videos
Nucleic Acid Structure
DNA Structure
DNA has a double-helix structure. The...
The Replisome
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
DNA Replication
Replication in Prokaryotes
DNA replication uses a large number of...
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...
Sanger Sequencing
ATP and Macromolecule Synthesis
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...

